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KMID : 0880220080460050491
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Journal of Microbiology
2008 Volume.46 No. 5 p.491 ~ p.501
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Bacterial, Archaeal, and Eukaryal Diversity in the Intestines of Korean People
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Nam Young-Do
Kim Min-Soo
Yoon Jung-Hoon
Kim Jong-Yeol
Lee Si-Woo
Kim Kyoung-Ho
Chang Ho-Won
Roh Seong-Woon
Bae Jin-Woo
Jung Mi-Ja
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Abstract
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The bacterial, archaeal, and eukaryal diversity in fecal samples from ten Koreans were analyzed and compared by using the PCR-fingerprinting method, denaturing gradient gel electrophoresis (DGGE). The bacteria all belonged to the Firmicutes and Bacteroidetes phyla, which were known to be the dominant bacterial species in the human intestine. Most of the archaeal sequences belonged to the methane-producing archaea but several halophilic archarea-related sequences were also detected unexpectedly. While a small number of eukaryal sequences were also detected upon DGGE analysis, these sequences were related to fungi and stramenopiles (Blastocystis hominis). With regard to the bacterial and archaeal DGGE analysis, all ten samples had one and two prominent bands, respectively, but many individual-specific bands were also observed. However, only five of the ten samples had small eukaryal DGGE bands and none of these bands was observed in all five samples. Unweighted pair group method and arithmetic averages clustering algorithm (UPGMA) clustering analysis revealed that the archaeal and bacterial communities in the ten samples had relatively higher relatedness (the average Dice coefficient values were 68.9 and 59.2% for archaea and bacteria, respectively) but the eukaryal community showed low relatedness (39.6%).
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KEYWORD
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human intestinal microbes, Bacteria, Archaea, Eukaryote, DGGE, UPGMA
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